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hek 293t humanace2  (ATCC)


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    ATCC hek 293t humanace2
    Hek 293t Humanace2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 37978 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hek+293t+humanace2/293T/pm35303475-264-192-201
    Average 99 stars, based on 37978 article reviews
    hek 293t humanace2 - by Bioz Stars, 2026-09
    99/100 stars

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    Isolation:

    Article Title: Rationally designed immunogens enable immune focusing following SARS-CoV-2 spike imprinting.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CR3022 Produced in house (Yuan et al., 2020) N/A S309 Produced in house (Pinto et al., 2020 N/A P2B-2F6 Produced in house (Ju et al., 2020 N/A B38 Produced in house (Wu et al., 2020a, 2020b) N/A ADI-56046 Produced in house (Wec et al., 2020 N/A ADI-55689 Produced in house (Wec et al., 2020) N/A ADI-55688 Produced in house (Wec et al., 2020 N/A Ab15 This paper N/A Ab16 This paper N/A Ab17 This paper N/A Ab19 This paper N/A Ab20 This paper N/A HRP-conjugated rabbit anti-mouse IgG antibody Abcam CAT#ab97046 HRP-conjugated goat anti-mouse IgG, human/bovine/horse SP ads antibody Southern Biotech CAT#1013-05 CD3-BV786 BioLegend CAT#100232 CD19-BV421 BioLegend CAT#115549 IgM-BV605 BioLegend CAT#406523 IgG-PerCP/Cy5.5 BioLegend CAT#405314 Bacterial and virus strains Sarbecovirus pseudotyped viruses Produced in house (Garcia-Beltran et al., 2021a, 2021b) N/A WA1/2020 SARS-CoV-2 strain with a D614G mutation (Chen et al., 2021) N/A Chemicals, peptides, and recombinant proteins Monomeric and trimeric SARS-2, SARS-1, and WIV1 receptor binding domains This paper N/A Monomeric SHC014 and RaTG13 receptor binding domains This paper N/A SARS-2 two-proline stabilized spike ectodomain Produced in house (Wrapp et al., 2020) N/A SARS-2hg monomer and trimer This paper N/A rsSARS-1hg monomer and trimer This paper N/A rsWIV1hg monomer and trimer This paper N/A RBMhg monomer and trimer This paper N/A Trimeric hemagglutinin head This paper N/A Pierce HRV 3C protease ThermoScientific CAT#88946 Sigma Adjuvant System SigmaAldrich CAT#S6322 Streptavidin-PE conjugate Invitrogen CAT#12-4317-87 Aqua Live/Dead amine-reactive dye ThermoFisher CAT#L34957 Calcein, AM, cell-permeant dye ThermoFisher CAT#C3100MP Streptavidin-APC/Cy7 conjugate BioLegend CAT#405208 Streptavidin-BV650 conjugate BioLegend CAT#405232 StrepTactin-PE conjugate IBA Lifesciences Item#6-5000-001 (Continued on next page) e1 Cell Reports 38, 110561, March 22, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER StrepTactin-APC conjugate IBA Lifesciences Item#6-5010-001 RNaseOUT ThermoFisher CAT#10777019 TALON Metal Affinity Resin Takara CAT#635652 Pierce Protein G Agarose ThermoFisher CAT#20399 1-Step ABTS substrate ThermoFisher Prod#37615 Critical commercial assays FAB2G Biosensors ForteBio Item#18-5125 Ni-NTA Biosensors ForteBio Item#18-5101 SuperScript IV VILO MasterMix ThermoFisher CAT#11756050 Index crystal screen Hampton Research Item#HR2-144 MagMax mirVana Total RNA isolation kit ThermoFisher CAT#A27828 Deposited data Atomic coordinates, Ab17 and SARS-2 RBD complex Protein Data Bank PDB 7TE1 Electron density map, Ab16 and SARS-2 spike complex Electron Microscopy Data Bank EMD-24894 Electron density map, Ab20 and SARS-2 spike complex Electron Microscopy Data Bank EMD-24895 Ab15 variable heavy chain Genbank OM407408 Ab16 variable heavy chain Genbank OM407409 Ab17 variable heavy chain Genbank OM407410 Ab19 variable heavy chain Genbank OM407411 Ab20 variable heavy chain Genbank OM407412 Ab15 variable light chain Genbank OM407413 Ab16 variable light chain Genbank OM407414 Ab17 variable light chain Genbank OM407415 Ab19 variable light chain Genbank OM407416 Ab20 variable light chain Genbank OM407417 B cell receptor sequences Genbank See Table S4 for accession numbers Experimental models: Cell lines Human: FreeStyle 293F Thermo Fisher Cat#R79007; RRID: CVCL_D603 Human: Expi293F Thermo Fisher Cat#A14527; RRID: CVCL_D615 Human: HEK 293T-humanACE2 (Moore et al., 2004) Human: HEK293S GnTI-/- ATCC ATCC CRL-3022 Experimental models: Organisms/strains C57BL/6 mice (strain: C57BL/6NCrl) Charles River Laboratories Strain code: 027 K18-hACE2 C57BL/6J mice (strain: 2B6.Cg-Tg(K18-ACE2)2Prlmn/J) Jackson Laboratory Strain #034860 Oligonucleotides Murine B cell receptor sequencing primers (Rohatgi et al., 2008; Tiller et al., 2009) See Table S3 for oligo sequences Software and algorithms FlowJo v10 TreeStar https://www.flowjo.com; RRID: SCR_008520 Prism v9 GraphPad https://www.graphpad.com; RRID: SCR_002798 IMGT International ImMunoGeneTics Information System http://www.imgt.org; RRID: SCR_012780 Cloanalyst (Kepler et al., 2014) https://www.bu.edu/ computationalimmunology/research/ software/ (Continued on next page) Cell Reports 38, 110561, March 22, 2022 e2 ..

    Electron Microscopy:

    Article Title: Rationally designed immunogens enable immune focusing following SARS-CoV-2 spike imprinting.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CR3022 Produced in house (Yuan et al., 2020) N/A S309 Produced in house (Pinto et al., 2020 N/A P2B-2F6 Produced in house (Ju et al., 2020 N/A B38 Produced in house (Wu et al., 2020a, 2020b) N/A ADI-56046 Produced in house (Wec et al., 2020 N/A ADI-55689 Produced in house (Wec et al., 2020) N/A ADI-55688 Produced in house (Wec et al., 2020 N/A Ab15 This paper N/A Ab16 This paper N/A Ab17 This paper N/A Ab19 This paper N/A Ab20 This paper N/A HRP-conjugated rabbit anti-mouse IgG antibody Abcam CAT#ab97046 HRP-conjugated goat anti-mouse IgG, human/bovine/horse SP ads antibody Southern Biotech CAT#1013-05 CD3-BV786 BioLegend CAT#100232 CD19-BV421 BioLegend CAT#115549 IgM-BV605 BioLegend CAT#406523 IgG-PerCP/Cy5.5 BioLegend CAT#405314 Bacterial and virus strains Sarbecovirus pseudotyped viruses Produced in house (Garcia-Beltran et al., 2021a, 2021b) N/A WA1/2020 SARS-CoV-2 strain with a D614G mutation (Chen et al., 2021) N/A Chemicals, peptides, and recombinant proteins Monomeric and trimeric SARS-2, SARS-1, and WIV1 receptor binding domains This paper N/A Monomeric SHC014 and RaTG13 receptor binding domains This paper N/A SARS-2 two-proline stabilized spike ectodomain Produced in house (Wrapp et al., 2020) N/A SARS-2hg monomer and trimer This paper N/A rsSARS-1hg monomer and trimer This paper N/A rsWIV1hg monomer and trimer This paper N/A RBMhg monomer and trimer This paper N/A Trimeric hemagglutinin head This paper N/A Pierce HRV 3C protease ThermoScientific CAT#88946 Sigma Adjuvant System SigmaAldrich CAT#S6322 Streptavidin-PE conjugate Invitrogen CAT#12-4317-87 Aqua Live/Dead amine-reactive dye ThermoFisher CAT#L34957 Calcein, AM, cell-permeant dye ThermoFisher CAT#C3100MP Streptavidin-APC/Cy7 conjugate BioLegend CAT#405208 Streptavidin-BV650 conjugate BioLegend CAT#405232 StrepTactin-PE conjugate IBA Lifesciences Item#6-5000-001 (Continued on next page) e1 Cell Reports 38, 110561, March 22, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER StrepTactin-APC conjugate IBA Lifesciences Item#6-5010-001 RNaseOUT ThermoFisher CAT#10777019 TALON Metal Affinity Resin Takara CAT#635652 Pierce Protein G Agarose ThermoFisher CAT#20399 1-Step ABTS substrate ThermoFisher Prod#37615 Critical commercial assays FAB2G Biosensors ForteBio Item#18-5125 Ni-NTA Biosensors ForteBio Item#18-5101 SuperScript IV VILO MasterMix ThermoFisher CAT#11756050 Index crystal screen Hampton Research Item#HR2-144 MagMax mirVana Total RNA isolation kit ThermoFisher CAT#A27828 Deposited data Atomic coordinates, Ab17 and SARS-2 RBD complex Protein Data Bank PDB 7TE1 Electron density map, Ab16 and SARS-2 spike complex Electron Microscopy Data Bank EMD-24894 Electron density map, Ab20 and SARS-2 spike complex Electron Microscopy Data Bank EMD-24895 Ab15 variable heavy chain Genbank OM407408 Ab16 variable heavy chain Genbank OM407409 Ab17 variable heavy chain Genbank OM407410 Ab19 variable heavy chain Genbank OM407411 Ab20 variable heavy chain Genbank OM407412 Ab15 variable light chain Genbank OM407413 Ab16 variable light chain Genbank OM407414 Ab17 variable light chain Genbank OM407415 Ab19 variable light chain Genbank OM407416 Ab20 variable light chain Genbank OM407417 B cell receptor sequences Genbank See Table S4 for accession numbers Experimental models: Cell lines Human: FreeStyle 293F Thermo Fisher Cat#R79007; RRID: CVCL_D603 Human: Expi293F Thermo Fisher Cat#A14527; RRID: CVCL_D615 Human: HEK 293T-humanACE2 (Moore et al., 2004) Human: HEK293S GnTI-/- ATCC ATCC CRL-3022 Experimental models: Organisms/strains C57BL/6 mice (strain: C57BL/6NCrl) Charles River Laboratories Strain code: 027 K18-hACE2 C57BL/6J mice (strain: 2B6.Cg-Tg(K18-ACE2)2Prlmn/J) Jackson Laboratory Strain #034860 Oligonucleotides Murine B cell receptor sequencing primers (Rohatgi et al., 2008; Tiller et al., 2009) See Table S3 for oligo sequences Software and algorithms FlowJo v10 TreeStar https://www.flowjo.com; RRID: SCR_008520 Prism v9 GraphPad https://www.graphpad.com; RRID: SCR_002798 IMGT International ImMunoGeneTics Information System http://www.imgt.org; RRID: SCR_012780 Cloanalyst (Kepler et al., 2014) https://www.bu.edu/ computationalimmunology/research/ software/ (Continued on next page) Cell Reports 38, 110561, March 22, 2022 e2 ..

    Sequencing:

    Article Title: Rationally designed immunogens enable immune focusing following SARS-CoV-2 spike imprinting.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CR3022 Produced in house (Yuan et al., 2020) N/A S309 Produced in house (Pinto et al., 2020 N/A P2B-2F6 Produced in house (Ju et al., 2020 N/A B38 Produced in house (Wu et al., 2020a, 2020b) N/A ADI-56046 Produced in house (Wec et al., 2020 N/A ADI-55689 Produced in house (Wec et al., 2020) N/A ADI-55688 Produced in house (Wec et al., 2020 N/A Ab15 This paper N/A Ab16 This paper N/A Ab17 This paper N/A Ab19 This paper N/A Ab20 This paper N/A HRP-conjugated rabbit anti-mouse IgG antibody Abcam CAT#ab97046 HRP-conjugated goat anti-mouse IgG, human/bovine/horse SP ads antibody Southern Biotech CAT#1013-05 CD3-BV786 BioLegend CAT#100232 CD19-BV421 BioLegend CAT#115549 IgM-BV605 BioLegend CAT#406523 IgG-PerCP/Cy5.5 BioLegend CAT#405314 Bacterial and virus strains Sarbecovirus pseudotyped viruses Produced in house (Garcia-Beltran et al., 2021a, 2021b) N/A WA1/2020 SARS-CoV-2 strain with a D614G mutation (Chen et al., 2021) N/A Chemicals, peptides, and recombinant proteins Monomeric and trimeric SARS-2, SARS-1, and WIV1 receptor binding domains This paper N/A Monomeric SHC014 and RaTG13 receptor binding domains This paper N/A SARS-2 two-proline stabilized spike ectodomain Produced in house (Wrapp et al., 2020) N/A SARS-2hg monomer and trimer This paper N/A rsSARS-1hg monomer and trimer This paper N/A rsWIV1hg monomer and trimer This paper N/A RBMhg monomer and trimer This paper N/A Trimeric hemagglutinin head This paper N/A Pierce HRV 3C protease ThermoScientific CAT#88946 Sigma Adjuvant System SigmaAldrich CAT#S6322 Streptavidin-PE conjugate Invitrogen CAT#12-4317-87 Aqua Live/Dead amine-reactive dye ThermoFisher CAT#L34957 Calcein, AM, cell-permeant dye ThermoFisher CAT#C3100MP Streptavidin-APC/Cy7 conjugate BioLegend CAT#405208 Streptavidin-BV650 conjugate BioLegend CAT#405232 StrepTactin-PE conjugate IBA Lifesciences Item#6-5000-001 (Continued on next page) e1 Cell Reports 38, 110561, March 22, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER StrepTactin-APC conjugate IBA Lifesciences Item#6-5010-001 RNaseOUT ThermoFisher CAT#10777019 TALON Metal Affinity Resin Takara CAT#635652 Pierce Protein G Agarose ThermoFisher CAT#20399 1-Step ABTS substrate ThermoFisher Prod#37615 Critical commercial assays FAB2G Biosensors ForteBio Item#18-5125 Ni-NTA Biosensors ForteBio Item#18-5101 SuperScript IV VILO MasterMix ThermoFisher CAT#11756050 Index crystal screen Hampton Research Item#HR2-144 MagMax mirVana Total RNA isolation kit ThermoFisher CAT#A27828 Deposited data Atomic coordinates, Ab17 and SARS-2 RBD complex Protein Data Bank PDB 7TE1 Electron density map, Ab16 and SARS-2 spike complex Electron Microscopy Data Bank EMD-24894 Electron density map, Ab20 and SARS-2 spike complex Electron Microscopy Data Bank EMD-24895 Ab15 variable heavy chain Genbank OM407408 Ab16 variable heavy chain Genbank OM407409 Ab17 variable heavy chain Genbank OM407410 Ab19 variable heavy chain Genbank OM407411 Ab20 variable heavy chain Genbank OM407412 Ab15 variable light chain Genbank OM407413 Ab16 variable light chain Genbank OM407414 Ab17 variable light chain Genbank OM407415 Ab19 variable light chain Genbank OM407416 Ab20 variable light chain Genbank OM407417 B cell receptor sequences Genbank See Table S4 for accession numbers Experimental models: Cell lines Human: FreeStyle 293F Thermo Fisher Cat#R79007; RRID: CVCL_D603 Human: Expi293F Thermo Fisher Cat#A14527; RRID: CVCL_D615 Human: HEK 293T-humanACE2 (Moore et al., 2004) Human: HEK293S GnTI-/- ATCC ATCC CRL-3022 Experimental models: Organisms/strains C57BL/6 mice (strain: C57BL/6NCrl) Charles River Laboratories Strain code: 027 K18-hACE2 C57BL/6J mice (strain: 2B6.Cg-Tg(K18-ACE2)2Prlmn/J) Jackson Laboratory Strain #034860 Oligonucleotides Murine B cell receptor sequencing primers (Rohatgi et al., 2008; Tiller et al., 2009) See Table S3 for oligo sequences Software and algorithms FlowJo v10 TreeStar https://www.flowjo.com; RRID: SCR_008520 Prism v9 GraphPad https://www.graphpad.com; RRID: SCR_002798 IMGT International ImMunoGeneTics Information System http://www.imgt.org; RRID: SCR_012780 Cloanalyst (Kepler et al., 2014) https://www.bu.edu/ computationalimmunology/research/ software/ (Continued on next page) Cell Reports 38, 110561, March 22, 2022 e2 ..

    Software:

    Article Title: Rationally designed immunogens enable immune focusing following SARS-CoV-2 spike imprinting.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CR3022 Produced in house (Yuan et al., 2020) N/A S309 Produced in house (Pinto et al., 2020 N/A P2B-2F6 Produced in house (Ju et al., 2020 N/A B38 Produced in house (Wu et al., 2020a, 2020b) N/A ADI-56046 Produced in house (Wec et al., 2020 N/A ADI-55689 Produced in house (Wec et al., 2020) N/A ADI-55688 Produced in house (Wec et al., 2020 N/A Ab15 This paper N/A Ab16 This paper N/A Ab17 This paper N/A Ab19 This paper N/A Ab20 This paper N/A HRP-conjugated rabbit anti-mouse IgG antibody Abcam CAT#ab97046 HRP-conjugated goat anti-mouse IgG, human/bovine/horse SP ads antibody Southern Biotech CAT#1013-05 CD3-BV786 BioLegend CAT#100232 CD19-BV421 BioLegend CAT#115549 IgM-BV605 BioLegend CAT#406523 IgG-PerCP/Cy5.5 BioLegend CAT#405314 Bacterial and virus strains Sarbecovirus pseudotyped viruses Produced in house (Garcia-Beltran et al., 2021a, 2021b) N/A WA1/2020 SARS-CoV-2 strain with a D614G mutation (Chen et al., 2021) N/A Chemicals, peptides, and recombinant proteins Monomeric and trimeric SARS-2, SARS-1, and WIV1 receptor binding domains This paper N/A Monomeric SHC014 and RaTG13 receptor binding domains This paper N/A SARS-2 two-proline stabilized spike ectodomain Produced in house (Wrapp et al., 2020) N/A SARS-2hg monomer and trimer This paper N/A rsSARS-1hg monomer and trimer This paper N/A rsWIV1hg monomer and trimer This paper N/A RBMhg monomer and trimer This paper N/A Trimeric hemagglutinin head This paper N/A Pierce HRV 3C protease ThermoScientific CAT#88946 Sigma Adjuvant System SigmaAldrich CAT#S6322 Streptavidin-PE conjugate Invitrogen CAT#12-4317-87 Aqua Live/Dead amine-reactive dye ThermoFisher CAT#L34957 Calcein, AM, cell-permeant dye ThermoFisher CAT#C3100MP Streptavidin-APC/Cy7 conjugate BioLegend CAT#405208 Streptavidin-BV650 conjugate BioLegend CAT#405232 StrepTactin-PE conjugate IBA Lifesciences Item#6-5000-001 (Continued on next page) e1 Cell Reports 38, 110561, March 22, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER StrepTactin-APC conjugate IBA Lifesciences Item#6-5010-001 RNaseOUT ThermoFisher CAT#10777019 TALON Metal Affinity Resin Takara CAT#635652 Pierce Protein G Agarose ThermoFisher CAT#20399 1-Step ABTS substrate ThermoFisher Prod#37615 Critical commercial assays FAB2G Biosensors ForteBio Item#18-5125 Ni-NTA Biosensors ForteBio Item#18-5101 SuperScript IV VILO MasterMix ThermoFisher CAT#11756050 Index crystal screen Hampton Research Item#HR2-144 MagMax mirVana Total RNA isolation kit ThermoFisher CAT#A27828 Deposited data Atomic coordinates, Ab17 and SARS-2 RBD complex Protein Data Bank PDB 7TE1 Electron density map, Ab16 and SARS-2 spike complex Electron Microscopy Data Bank EMD-24894 Electron density map, Ab20 and SARS-2 spike complex Electron Microscopy Data Bank EMD-24895 Ab15 variable heavy chain Genbank OM407408 Ab16 variable heavy chain Genbank OM407409 Ab17 variable heavy chain Genbank OM407410 Ab19 variable heavy chain Genbank OM407411 Ab20 variable heavy chain Genbank OM407412 Ab15 variable light chain Genbank OM407413 Ab16 variable light chain Genbank OM407414 Ab17 variable light chain Genbank OM407415 Ab19 variable light chain Genbank OM407416 Ab20 variable light chain Genbank OM407417 B cell receptor sequences Genbank See Table S4 for accession numbers Experimental models: Cell lines Human: FreeStyle 293F Thermo Fisher Cat#R79007; RRID: CVCL_D603 Human: Expi293F Thermo Fisher Cat#A14527; RRID: CVCL_D615 Human: HEK 293T-humanACE2 (Moore et al., 2004) Human: HEK293S GnTI-/- ATCC ATCC CRL-3022 Experimental models: Organisms/strains C57BL/6 mice (strain: C57BL/6NCrl) Charles River Laboratories Strain code: 027 K18-hACE2 C57BL/6J mice (strain: 2B6.Cg-Tg(K18-ACE2)2Prlmn/J) Jackson Laboratory Strain #034860 Oligonucleotides Murine B cell receptor sequencing primers (Rohatgi et al., 2008; Tiller et al., 2009) See Table S3 for oligo sequences Software and algorithms FlowJo v10 TreeStar https://www.flowjo.com; RRID: SCR_008520 Prism v9 GraphPad https://www.graphpad.com; RRID: SCR_002798 IMGT International ImMunoGeneTics Information System http://www.imgt.org; RRID: SCR_012780 Cloanalyst (Kepler et al., 2014) https://www.bu.edu/ computationalimmunology/research/ software/ (Continued on next page) Cell Reports 38, 110561, March 22, 2022 e2 ..



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    hek 293t humanace2  (ATCC)
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    ATCC hek 293t humanace2
    Hek 293t Humanace2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hek+293t+humanace2/293T/pm35303475-264-192-201
    Average 99 stars, based on 1 article reviews
    hek 293t humanace2 - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

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